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Rabbit Monoclonal Epac1 Antibody

  • 中文名： Epac1抗体
  • 别    名： RAPGEF3; CGEF1; EPAC; EPAC1; Rap guanine nucleotide exchange factor 3; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; EPAC 1; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP
货号: IPDX21359
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesWSS; NFNS; VRNF
WB Predicted band size319 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human NF1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于Epac1抗体的3-4条参考文献示例(基于已有研究领域常见主题，具体文献可能需要进一步验证)：

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1. **"Epac1-Mediated Signaling in Pancreatic β-Cell Function"**

*Authors: K. K. H. Cheng et al.*

**摘要**：本研究利用Epac1特异性抗体，通过Western blot和免疫荧光技术，揭示了Epac1在cAMP调控的胰岛素分泌中的关键作用，表明其激活可增强β细胞功能。

2. **"Epac1 Drives Cardiac Hypertrophy via cAMP-Dependent Pathways"**

*Authors: C. P. Nelson et al.*

**摘要**：通过Epac1抗体检测心脏组织中的蛋白表达，研究发现Epac1的过度激活通过Rap1信号通路促进病理性心肌肥大，提示其作为潜在治疗靶点。

3. **"Epac1 Promotes Tumor Cell Invasion via Matrix Metalloproteinase Activation"**

*Authors: F. Ribeiro-Neto et al.*

**摘要**：采用Epac1抗体进行免疫沉淀和免疫组化实验，证明Epac1通过cAMP依赖的MMP-9上调增强癌细胞迁移和侵袭，与肿瘤转移相关。

4. **"Epac1 in Neuronal Axon Guidance: A Role in cAMP Signaling"**

*Authors: R. G. Pereira et al.*

**摘要**：通过Epac1抗体标记及基因敲除模型，发现Epac1参与神经元轴突导向，调控生长锥对导向分子的响应，影响神经发育。

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**注**：以上文献为示例性质，实际引用时建议通过PubMed或Google Scholar检索具体文献(关键词：Epac1 antibody, cAMP signaling, [研究领域])。知名Epac1抗体供应商(如Santa Cruz、Cell Signaling Technology)的产品说明书也可能引用相关文献。

背景信息

Epac1 (Exchange protein directly activated by cAMP 1), also known as RAPGEF3. is a key intracellular sensor of cyclic AMP (cAMP) that mediates cAMP signaling independently of protein kinase A (PKA). It functions as a guanine nucleotide exchange factor (GEF) for Rap GTPases, regulating diverse physiological processes such as cell adhesion, secretion, and differentiation. Epac1 antibodies are essential tools for studying the expression, localization, and function of Epac1 in various tissues and disease models. These antibodies are typically developed using immunogenic peptides or recombinant Epac1 protein regions, enabling specific detection of Epac1 isoforms (∼100 kDa) in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Due to structural similarities between Epac1 and Epac2 (a homologous isoform), antibody specificity is rigorously validated using knockout controls or siRNA-mediated silencing. Epac1 antibodies have been instrumental in uncovering the protein’s role in cardiovascular diseases, diabetes, and cancer, particularly in pathways involving cAMP-mediated cell signaling. Commercial Epac1 antibodies are available from multiple suppliers, with validation data often including reactivity across human, mouse, and rat samples. Researchers prioritize antibodies with minimal cross-reactivity to Epac2 or other cAMP-binding proteins to ensure experimental accuracy.

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