| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Epsin 1 |
| Entrez GeneID | 29924 |
| WB Predicted band size | Calculated MW: 60 kDa; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Epsin 1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Epsin 1抗体的3篇参考文献示例(注：以下内容为示例性概括，具体文献需根据实际检索补充)：
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1. **文献名称**：*Epsin 1 is a polyubiquitin-selective clathrin adaptor whose ligand endocytosis is autoinhibited by its C-terminal region*
**作者**：Ford, M.G. et al.
**摘要**：该研究阐明了Epsin 1通过其N端ENTH结构域结合脂膜，并利用泛素结合基序(UBAs)识别多聚泛素化受体，促进网格蛋白介导的内吞作用。研究中使用特异性Epsin 1抗体进行免疫印迹和免疫沉淀实验，验证了其与泛素化蛋白的相互作用。
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2. **文献名称**：*Epsin family proteins regulate membrane curvature through their N-terminal ENTH domain*
**作者**：Mishra, S.K. et al.
**摘要**：本文研究了Epsin蛋白家族(包括Epsin 1)通过ENTH结构域诱导膜曲率的功能。通过Epsin 1抗体的免疫荧光染色，作者观察到其在细胞膜内吞位点的定位，并证实其参与网格蛋白包被囊泡的形成。
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3. **文献名称**：*Epsin 1 depletion attenuates tumor progression in colorectal cancer by modulating EGFR signaling*
**作者**：Chen, H. et al.
**摘要**：该研究探讨了Epsin 1在结直肠癌中通过调控EGFR内吞和信号传导促进肿瘤生长的机制。利用Epsin 1抗体进行免疫组化分析，发现其在癌组织中高表达，并与患者预后不良相关。
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4. **文献名称**：*Epsin 1 modulates synaptic vesicle retrieval and neurotransmitter release in neurons*
**作者**：Tomas, A. et al.
**摘要**：研究揭示了Epsin 1在神经元突触小泡回收中的作用。通过Epsin 1特异性抗体的Western blot和免疫电镜分析，证实其在小泡循环中的关键功能，并影响突触传递效率。
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**注意**：以上文献信息为模拟生成，实际引用需通过PubMed或Google Scholar检索真实文献(如PMID: 12345678等)。建议使用关键词“Epsin 1 antibody”或“Epsin 1 function”查询最新研究。
Epsin 1. a pivotal adaptor protein in clathrin-mediated endocytosis (CME), facilitates the internalization of ubiquitinated membrane receptors by linking ubiquitin signals to the endocytic machinery. It contains an N-terminal ENTH (epsin N-terminal homology) domain that binds phosphatidylinositol 4.5-bisphosphate (PIP2) to promote membrane curvature, and ubiquitin-binding domains (UBDs) that recognize ubiquitinated cargo. Epsin 1 also interacts with clathrin, AP-2. and other endocytic proteins via its C-terminal disordered regions, coordinating vesicle formation. Dysregulation of Epsin 1 is implicated in cancer, cardiovascular diseases, and neurological disorders due to its roles in receptor trafficking, signaling pathways (e.g., VEGF, Notch), and synaptic vesicle recycling.
Epsin 1 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate CME mechanisms, receptor downregulation, and disease-associated alterations. Polyclonal or monoclonal antibodies targeting specific domains (e.g., ENTH or UBD regions) help dissect structural-functional relationships. Recent studies also utilize Epsin 1 antibodies to explore its non-endocytic roles, such as modulating actin dynamics or DNA repair. Validated antibodies are critical for ensuring specificity, particularly given homology among epsin family members (Epsin 1-3). Overall, these antibodies advance research into cellular trafficking and pathological processes linked to endocytic dysregulation.
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