首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人甲状腺过氧化物酶(TPO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TPO含量。TPO即血小板生成素，是刺激巨核细胞生长及分化的内源性细胞因子。它能促进巨核细胞的增殖、分化以及血小板的生成释放。在研究中，其机制主要围绕与受体结合激活信号传导通路，为血小板减少症等治疗研究提供了方向。试剂盒检测范围为0.9 ng/mL-60 ng/mL，本产品为科研人员提供可靠的实验工具，可用于探索甲状腺功能调控机制、评估疾病模型动物体内TPO水平变化，或作为体外实验的定量分析手段，为甲状腺相关疾病的分子机制研究提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MSA ELISA Kit; PERT_HUMAN ELISA Kit; TDH2A ELISA Kit; Thyroid microsomal antigen ELISA Kit; Thyroid peroxidase ELISA Kit; Thyroperoxidase ELISA Kit; TPO ELISA Kit; TPX ELISA Kit
缩写：TPO
Uniprot No.：P07202
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.9 ng/mL-60 ng/mL
灵敏度：0.225 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-103 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 85-96 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 85-99 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 87-106 | ||||||
回收率：
| The recovery of human TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 82-102 | |||||
| EDTA plasma (n=4) | 92 | 88-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected602.2442.2372.2412.103301.9851.9921.9891.851151.6081.6131.6111.4737.51.1371.1551.1461.0083.750.7020.7140.7080.5701.80.5060.5120.5090.3710.90.3510.3590.3550.21700.1370.1390.138 | |||||||
货期：3-5 working days
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