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产品描述：人原肌球蛋白α4(TPM4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TPM4含量。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于基础科研中TPM4蛋白表达水平的定量分析，包括但不限于肿瘤细胞侵袭转移机制研究、心肌功能异常模型分析、骨骼肌疾病病理探索，以及细胞骨架动态变化或相关信号通路的功能研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：epididymis secretory protein Li 108 ELISA Kit; HEL-S-108 ELISA Kit; TM30p1 ELISA Kit; TMP 4 ELISA Kit; TPM4 ELISA Kit; TPM4_HUMAN ELISA Kit; Tropomyosin 4 ELISA Kit; Tropomyosin alpha 4 chain ELISA Kit; Tropomyosin alpha-4 chain ELISA Kit; Tropomyosin-4 ELISA Kit; wu:fb06e07 ELISA Kit; zgc:103436 ELISA Kit; zgc:63909 ELISA Kit
缩写：TPM4
Uniprot No.：P67936
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TPM4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-104 | |||||
回收率：
| The recovery of human TPM4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-90 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.5572.5972.5772.3987501.9161.9361.9261.7473751.2781.2981.2881.109187.50.7980.7420.7700.591940.4810.4990.4900.311470.3670.3550.3610.18223.50.2640.2680.2660.08700.1780.1790.179 | ||||||
货期：3-5 working days
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