| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E |
| Entrez GeneID | 10049 |
| WB Predicted band size | Calculated MW: 36 kDa; Observed MW: 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human DNAJB6 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4条关于DNAJB6抗体的参考文献及其简要摘要：
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1. **文献名称**：*DNAJB6 regulates a thermoprotective Hsp70/Hsp90 chaperone network in stress granules*
**作者**：Walters RW, et al.
**摘要**：该研究探讨DNAJB6在应激颗粒中的作用，利用特异性抗体验证其与Hsp70/Hsp90的相互作用，揭示其通过调控分子伴侣网络增强细胞在热应激下的生存能力。
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2. **文献名称**：*DNAJB6 is a peptide-binding chaperone which can suppress amyloid fibrillation of polyglutamine peptides*
**作者**：Hageman J, et al.
**摘要**：研究通过免疫印迹和免疫沉淀实验(使用DNAJB6抗体)，证明DNAJB6通过结合多聚谷氨酰胺肽抑制淀粉样蛋白聚集，为神经退行性疾病治疗提供机制见解。
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3. **文献名称**：*Mutations in DNAJB6 cause dominant limb-girdle muscular dystrophy*
**作者**：Bengoechea R, et al.
**摘要**：该文献利用DNAJB6抗体进行免疫荧光和Western blot分析，发现DNAJB6突变导致其分子伴侣功能受损，引发肢带型肌营养不良症，提示其在肌肉蛋白质量控制中的关键作用。
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4. **文献名称**：*Structural basis of the interaction between Hsp90 and DNAJB6 in amyloid fibril suppression*
**作者**：Kampinga HH, et al.
**摘要**：通过抗体验证DNAJB6与Hsp90的互作，结合冷冻电镜技术解析复合物结构，阐明DNAJB6通过协同Hsp90抑制β淀粉样蛋白纤维形成的分子机制。
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这些文献均涉及DNAJB6抗体的应用，涵盖功能研究、疾病机制及结构解析，适合作为相关研究的参考依据。
DNAJB6 is a member of the HSP40/DnaJ protein family, which functions as a co-chaperone for HSP70 to regulate protein folding, degradation, and aggregation. It plays a critical role in maintaining proteostasis, particularly in preventing the aggregation of misfolded proteins linked to neurodegenerative diseases such as Alzheimer’s and Parkinson’s. DNAJB6 exists in two major isoforms: a cytoplasmic form (DNAJB6a) and a nuclear form (DNAJB6b), with distinct cellular localization and functions. Mutations in DNAJB6 are associated with limb-girdle muscular dystrophy type 1D (LGMD1D), highlighting its importance in muscle integrity.
Antibodies targeting DNAJB6 are essential tools for studying its expression, localization, and interactions in both physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate DNAJB6’s role in cellular stress responses, cancer progression (where it may act as a tumor suppressor or promoter), and neuromuscular disorders. Commercially available DNAJB6 antibodies are typically raised against specific epitopes of human or murine DNAJB6. with validation across species and applications. Researchers must select antibodies based on isoform specificity, cross-reactivity, and compatibility with experimental models. These reagents contribute to understanding DNAJB6’s dual roles in proteostasis and disease, offering potential insights for therapeutic strategies targeting protein misfolding disorders or muscle degeneration.
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