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产品描述：人肌钙蛋白T，骨骼肌快肌(TNNT3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNNT3含量。TNNT3是重要靶点，它是心肌肌钙蛋白T的一种亚型。在研究机制上，它与心肌收缩功能紧密相关，其结构和功能变化会影响心肌收缩舒张。对它的深入研究有助于揭示心肌病等心脏疾病发病机制，为相关疾病诊断和治疗提供新方向。试剂盒检测范围为7.81 pg/mL-500 pg/mL，该产品可应用于运动科学领域研究运动后骨骼肌损伤的生物标志物动态变化，或用于肌肉疾病模型中TNNT3表达调控机制的探索，亦支持基因编辑细胞或动物模型的功能验证实验，为骨骼肌生理及病理机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AMCD2B ELISA Kit; Beta TnTF ELISA Kit; Beta-TnTF ELISA Kit; DA2B ELISA Kit; DKFZp779M2348 ELISA Kit; fast skeletal muscle ELISA Kit; Fast skeletal muscle troponin T ELISA Kit; FSSV ELISA Kit; fTnT ELISA Kit; TNNT 3 ELISA Kit; Tnnt3 ELISA Kit; TNNT3_HUMAN ELISA Kit; TnTf ELISA Kit; Troponin T ELISA Kit; Troponin T fast skeletal muscle ELISA Kit; Troponin T type 3 (skeletal fast) ELISA Kit; Troponin T type 3 ELISA Kit; Troponin T3 skeletal fast ELISA Kit
缩写：TNNT3
Uniprot No.：P45378
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.81 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TNNT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 89-101 | ||||||
| 1:2 | Average % | 107 | |||||
| Range % | 99-111 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 84-95 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 90-103 | ||||||
回收率：
| The recovery of human TNNT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 95-108 | |||||
| EDTA plasma (n=4) | 98 | 92-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5002.0331.9631.9981.8722501.7311.7781.7551.6291251.2551.2891.2721.14662.50.5960.6340.6150.48931.250.2970.3080.3030.17715.620.1890.1970.1930.0677.810.1530.1500.1520.02600.1280.1240.126 | |||||||
货期：3-5 working days
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