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Mouse Troponin T, cardiac muscle(TNNT2) ELISA kit

  • 中文名: 小鼠心肌特异性肌钙蛋白T(TNNT2)ELISA kit
货号: IPD-EL024016MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠心肌特异性肌钙蛋白T(TNNT2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的TNNT2含量。TNNT2是一个重要靶点。它是心肌肌钙蛋白T的编码基因,与心肌收缩等生理功能密切相关。在研究机制方面,其异常表达或突变可影响心肌功能,引发多种心脏疾病,目前针对该靶点在探索诊断标志物及治疗药物等。试剂盒检测范围为31.25 pg/ml-2000pg/ml,适用于科研领域中心肌损伤相关机制研究;适用于评估小鼠心肌损伤模型中的TNNT2动态变化,为心血管疾病机制探索、药物心脏毒性评价及转基因动物模型表型分析提供可靠工具,尤其适用于心肌梗死、心肌肥厚等病理过程中生物标志物的定量研究。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Tnnt2 ELISA Kit; Troponin T ELISA Kit; cardiac muscle ELISA Kit; TnTc ELISA Kit; Cardiac muscle troponin T ELISA Kit; cTnT ELISA Kit
  • 缩写:
    TNNT2
  • Uniprot No.:
    P50752
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    31.25 pg/ml-2000pg/ml
  • 灵敏度:
    7.8 pg/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNNT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    89-102


    1:2

    Average %

    91


    Range %

    82-98


    1:4

    Average %

    93


    Range %

    85-100


    1:8

    Average %

    92


    Range %

    88-100


  • 回收率:

    The recovery of mouse TNNT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    90

    90-100


    EDTA plasma (n=4)

    92

    87-96


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    2000

    1.912

    1.927

    1.920

    1.749


    1000

    1.513

    1.569

    1.541

    1.370


    500

    1.075

    1.138

    1.107

    0.936


    250

    0.804

    0.798

    0.801

    0.630


    125

    0.511

    0.537

    0.524

    0.353


    62.5

    0.349

    0.356

    0.353

    0.182


    31.25

    0.254

    0.245

    0.250

    0.079


    0

    0.172

    0.169

    0.171




  • 货期:
    3-5 working days

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