首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠丝氨酸/苏氨酸蛋白激酶TNNI3K(TNNI3K)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TNNI3K含量。TNNI3K 是一个重要靶点。它在心肌相关生理病理过程中发挥作用，其异常表达或功能变化可能与心肌疾病发生发展相关。研究机制上，主要探索其在心肌细胞信号转导、心肌重塑等方面机制，以寻找潜在治疗干预途径。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于科研场景下对大鼠心脏疾病模型、心肌细胞信号通路调控或药物干预实验中的TNNI3K动态表达分析，适用于体外实验中对不同来源生物样本的平行检测，为探索TNNI3K在心血管生理病理过程中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnni3k ELISA Kit; Cark ELISA Kit; Serine/threonine-protein kinase TNNI3K ELISA Kit; EC 2.7.11.1 ELISA Kit; Cardiac ankyrin repeat kinase ELISA Kit; TNNI3-interacting kinase ELISA Kit
缩写：TNNI3K
Uniprot No.：Q7TQP6
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.69 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TNNI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 91-103 | ||||||
回收率：
| The recovery of rat TNNI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-98 | |||||
| EDTA plasma (n=4) | 97 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected12002.4652.5612.5132.4016001.9321.8921.9121.8003001.2771.2981.2881.1761500.7080.7390.7240.612750.4120.4320.4220.31037.50.2870.2750.2810.16918.750.1830.1850.1840.07200.1110.1130.112 | |||||||
货期：3-5 working days
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