| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CK 7; CK-7; ck7; Cytokeratin 7; Cytokeratin-7; Cytokeratin7; D15Wsu77e; K2C7; K2C7_HUMAN; K7; Keratin 55k type ii cytoskeletal; Keratin 7; Keratin simple epithelial type 1 k7; Keratin type II cytoskeletal 7 |
| Entrez GeneID | 3855 |
| WB Predicted band size | Calculated MW: 51 kDa; Observed MW: 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Cytokeratin 7 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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1. **"Cytokeratin 7 and cytokeratin 20 in primary carcinoma of the liver"** by Chu P.G. et al.
- **摘要**: 探讨CK7和CK20在肝细胞癌和胆管癌中的表达差异，提出CK7阳性有助于鉴别胆管癌与其他肝脏原发肿瘤。
2. **"Immunohistochemical differentiation of metastatic breast carcinoma and metastatic ovarian carcinoma with anti-cytokeratin 7 and anti-cytokeratin 20 antibodies"** by Loy T.S. et al.
- **摘要**: 分析CK7和CK20在转移性乳腺癌与卵巢癌中的表达模式，表明CK7阳性/CK20阴性组合支持卵巢癌来源的诊断。
3. **"The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors, and cultured cells"** by Moll R. et al.
- **摘要**: 系统性描述CK7等细胞角蛋白在正常组织、肿瘤及细胞系中的分布，奠定其在肿瘤分型中的应用基础。
4. **"Utility of cytokeratin 7 and 20 in the differential diagnosis of primary and secondary adenocarcinomas of the lung"** by Kaufmann O. et al.
- **摘要**: 比较肺原发腺癌与转移性腺癌中CK7/CK20的表达差异，证实CK7在肺原发癌中高表达，支持其作为鉴别标记物。
Cytokeratin 7 (CK7) is a member of the cytokeratin family of intermediate filament proteins, which are essential structural components of epithelial cells. These proteins are classified into acidic Type I and basic Type II keratins, forming heteropolymers to maintain cellular integrity. CK7. a Type II keratin with a molecular weight of approximately 54 kDa, is expressed in specific epithelial tissues, including glandular and ductal epithelia of the respiratory, biliary, urinary tract, and endometrium.
In diagnostic pathology, CK7 antibodies are widely used in immunohistochemistry (IHC) to identify epithelial origin and differentiate between carcinomas. For instance, CK7 positivity is common in adenocarcinomas of the lung, breast, and ovary, while it is typically absent in colorectal, prostate, and hepatocellular carcinomas. This marker is often paired with CK20 to refine tumor classification (e.g., CK7+/CK20– in lung adenocarcinoma vs. CK7–/CK20+ in colorectal carcinoma).
CK7 antibodies also aid in detecting metastatic tumors of unknown primary origin by highlighting tissue-specific expression patterns. However, interpretation requires caution, as staining intensity and distribution may vary due to technical factors or tumor heterogeneity. Clinically, CK7 remains a cornerstone in differential diagnosis, though results should always correlate with clinical context and additional biomarkers.
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