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产品描述：鱼肿瘤坏死因子α(TNF-α)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的TNF-α含量。TNF-α，即肿瘤坏死因子-α，是一种重要的细胞因子，主要由活化的巨噬细胞产生。它通过与其受体TNFR1结合，激活多种信号通路，影响细胞增殖、凋亡和炎症反应。TNF-α在多种疾病如炎症、自身免疫性疾病中起关键作用，其研究有助于开发新的治疗策略。试剂盒检测范围为125 pg/mL-5000 pg/mL，本试剂盒适用于鱼类免疫相关研究，如病原感染模型中的炎症反应监测、疫苗免疫效果评估、水产养殖动物健康状态分析等科研场景，为水生生物免疫学机制研究及水产疾病防控提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TNF-α
Uniprot No.：Q8JFG3
种属：Fish
样本类型：serum, plasma
检测范围：125 pg/mL-5000 pg/mL
灵敏度：125 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-103 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of fish TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-102 | ||||
| EDTA plasma (n=4) | 95 | 91-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 50000.1540.1560.155 25000.3510.3280.340 11500.5620.5350.549 3750.8610.8640.863 1251.5441.5301.537 02.3872.3552.371 | ||||||
货期：3-5 working days
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