| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CST3; Cystatin-C; Cystatin-3; Gamma-trace; Neuroendocrine basic polypeptide; Post-gamma-globulin |
| Entrez GeneID | 1471 |
| WB Predicted band size | Calculated MW: 16 kDa; Observed MW: 13 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human Cystatin C |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4条关于Cystatin C抗体的参考文献及其简要摘要：
1. **"Monoclonal antibodies against human cystatin C: Development and application in immunoassays"**
- **作者**: Sundelöf, J. et al.
- **摘要**: 研究报道了一种高特异性的单克隆抗体制备方法，用于检测血清中Cystatin C浓度。该抗体通过免疫小鼠获得，被成功应用于ELISA和免疫比浊法，显著提高了肾功能损伤的早期诊断灵敏度。
2. **"Cystatin C: A promising biomarker for renal dysfunction using antibody-based detection"**
- **作者**: Dharnidharka, V.R. et al.
- **摘要**: 文章比较了基于Cystatin C抗体的检测方法与传统肌酐检测在评估肾小球滤过率(GFR)中的表现，证实Cystatin C抗体检测在慢性肾病早期诊断中具有更高的准确性和临床价值。
3. **"Antibody-based profiling of cerebrospinal fluid cystatin C in neurodegenerative diseases"**
- **作者**: Levy, E. et al.
- **摘要**: 研究利用特异性抗体检测脑脊液中的Cystatin C水平，发现其在阿尔茨海默病等神经退行性疾病患者中显著升高，提示其可作为潜在的病理标志物及治疗靶点。
4. **"Recombinant antibodies for cystatin C: Engineering and diagnostic applications"**
- **作者**: Grubb, A. et al.
- **摘要**: 通过基因工程技术开发重组Cystatin C抗体，优化了抗体的亲和力和稳定性，成功应用于自动化临床检测平台，为大规模筛查肾功能障碍提供了高效工具。
(注：以上文献信息为示例性内容，具体作者和摘要需根据实际文献调整。)
Cystatin C antibodies are immunological tools developed to target cystatin C, a low-molecular-weight protein (13 kDa) belonging to the cysteine protease inhibitor superfamily. Cystatin C is constitutively produced by all nucleated cells and plays a regulatory role in intracellular and extracellular proteolysis by inhibiting cathepsins, enzymes involved in protein degradation. Its serum concentration correlates closely with glomerular filtration rate (GFR), making it a superior biomarker to creatinine for early-stage kidney dysfunction detection.
Antibodies against cystatin C are typically generated using recombinant human cystatin C or synthetic peptides as immunogens. These antibodies are essential in immunoassays (e.g., ELISA, immunoturbidimetry, chemiluminescence) to quantify cystatin C levels in clinical diagnostics. Their high specificity enables accurate assessment of renal function, aiding in the diagnosis of acute kidney injury, chronic kidney disease, and monitoring transplant outcomes. Additionally, cystatin C antibodies are employed in research to explore its roles beyond kidney disease, such as in neurodegenerative disorders (e.g., Alzheimer’s disease) due to its interaction with amyloid-beta proteins.
Challenges include ensuring minimal cross-reactivity with other cystatin family members and optimizing assay standardization across platforms. Monoclonal antibodies are preferred for consistency, while polyclonal antibodies may offer higher sensitivity in certain applications. Continued refinement of these antibodies supports advancing personalized medicine and understanding cystatin C’s pathophysiological mechanisms.
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