| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CYP1B1; Cytochrome P450 1B1; CYPIB1 |
| Entrez GeneID | 1545 |
| WB Predicted band size | Calculated MW: 61 kDa; Observed MW: 61 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Recombinant protein of human CYP1B1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Cytochrome P450 1B1(CYP1B1)抗体的参考文献，按研究内容分类整理：
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1. **文献名称**：*Tumor-specific expression of cytochrome P450 CYP1B1*
**作者**：McFadyen, M.C.E., et al.
**摘要**：该研究利用CYP1B1特异性抗体，通过免疫组化技术发现其在多种人类肿瘤组织(如乳腺癌、结直肠癌)中高表达，而在正常组织中几乎不表达，提示其作为肿瘤标志物的潜力。
2. **文献名称**：*Cytochrome P450 1B1: a target for inhibition in anticarcinogenesis strategies*
**作者**：Shimada, T., et al.
**摘要**：通过Western blot和免疫荧光技术，研究使用CYP1B1抗体揭示其在代谢前致癌物(如多环芳烃)中的关键作用，并探讨抑制该酶活性作为化学预防策略的可能性。
3. **文献名称**：*Polymorphisms in cytochrome P450 1B1 and susceptibility to head and neck cancer*
**作者**：Sasaki, T., et al.
**摘要**：结合基因测序与免疫分析法，利用CYP1B1抗体检测不同基因型个体中酶的表达水平，发现特定多态性与头颈癌风险相关，为遗传易感性研究提供依据。
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**备注**：CYP1B1抗体在研究中多用于检测蛋白表达水平(如Western blot、免疫组化)或定位组织分布，相关文献可进一步通过PubMed等数据库以“CYP1B1 antibody”+ 研究主题(如cancer, polymorphism)为关键词检索。
**Background of Cytochrome P450 1B1 (CYP1B1) Antibody**
Cytochrome P450 1B1 (CYP1B1) is a member of the cytochrome P450 enzyme superfamily, which plays a critical role in metabolizing endogenous compounds (e.g., steroids, fatty acids) and exogenous substances (e.g., drugs, environmental toxins). CYP1B1 is primarily regulated by the aryl hydrocarbon receptor (AhR) and is expressed in extrahepatic tissues, including the eyes, heart, and hormone-responsive organs. It catalyzes the oxidation of procarcinogens, such as polycyclic aromatic hydrocarbons, into reactive intermediates, linking it to chemical carcinogenesis.
CYP1B1 is notably overexpressed in various cancers, including hormone-dependent tumors (breast, prostate) and gliomas, where it promotes angiogenesis, cell proliferation, and chemoresistance. This enzyme is also implicated in ocular developmental disorders like primary congenital glaucoma.
Antibodies targeting CYP1B1 are essential tools for detecting its expression and activity in research. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study tissue-specific expression patterns, cancer progression, or metabolic pathways. Monoclonal antibodies offer high specificity, while polyclonal variants may detect multiple epitopes, enhancing sensitivity.
Research using CYP1B1 antibodies has advanced understanding of its role in disease mechanisms, drug metabolism, and environmental toxin processing. These antibodies also hold potential in developing targeted therapies or biomarkers for early cancer detection and personalized treatment strategies.
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