| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cul2; CUL 2; CUL2; cullin 2 |
| Entrez GeneID | 8453 |
| WB Predicted band size | Calculated MW: 87 kDa; Observed MW: 87 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Cullin 2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Cullin 2抗体的参考文献(内容为虚构示例，仅作格式参考)：
1. **"Cullin 2 mediates VHL-dependent HIF-1α degradation in hypoxia signaling"**
- 作者：Maxwell, P.H. et al.
- 摘要：研究利用Cullin 2抗体证明其在Von Hippel-Lindau (VHL) E3泛素连接酶复合体中的核心作用，揭示Cul2通过招募底物HIF-1α并介导其氧依赖性泛素化降解的分子机制。
2. **"Structural analysis of Cullin 2-RING ligase assembly in ubiquitination"**
- 作者：Zheng, N. et al.
- 摘要：通过Cullin 2抗体的免疫共沉淀实验，解析了Cul2与Rbx1、Elongin B/C等蛋白的相互作用结构，阐明了其动态组装调控泛素化活性的分子基础。
3. **"Cullin 2 expression correlates with tumor progression in clear cell renal carcinoma"**
- 作者：Hickey, M.M. & Simon, M.C.
- 摘要：采用Cullin 2抗体进行组织染色，发现Cul2在肾透明细胞癌中高表达，且与HIF通路异常激活及患者预后不良显著相关，提示其作为潜在治疗靶点。
(注：以上文献名为虚构，如需真实文献建议通过PubMed或Google Scholar以“Cullin 2 antibody”、“CUL2 ubiquitin ligase”等关键词检索。)
Cullin 2 (CUL2) is a core component of the Cullin-RING ubiquitin ligase (CRL) complexes, which regulate protein ubiquitination and degradation. As a scaffold protein, CUL2 assembles with adaptors (e.g., Elongin B/C), substrate receptors (e.g., VHL protein), and a RING-domain protein (e.g., RBX1) to form functional E3 ligases. These complexes target specific substrates, such as hypoxia-inducible factor (HIF-1α), for proteasomal degradation, playing critical roles in oxygen sensing, cell cycle regulation, and tumor suppression. Dysregulation of CUL2-associated pathways is linked to cancers, including renal cell carcinoma and von Hippel-Lindau (VHL) disease.
CUL2 antibodies are essential tools for studying its expression, interactions, and functions in cellular processes. They are widely used in techniques like Western blotting, immunoprecipitation (IP), and immunofluorescence (IF) to detect CUL2 protein levels or map its localization. Researchers also employ these antibodies to investigate CRL complex assembly, substrate recognition, and post-translational modifications (e.g., neddylation). Validated CUL2 antibodies typically recognize the ~85 kDa protein and are tested for specificity via knockdown/knockout controls. Commercial antibodies often target conserved regions, such as the N-terminal domain, to ensure cross-reactivity in human, mouse, and rat models. Their applications extend to cancer research, drug discovery, and mechanistic studies of ubiquitin-proteasome system dynamics.
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