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产品描述：人肿瘤坏死因子α诱导蛋白3(TNFAIP3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFAIP3含量。TNFAIP3 是重要的靶点。它参与细胞内炎症与免疫调节等过程，可对肿瘤坏死因子等信号通路发挥负调控作用。研究其机制有助于理解免疫相关疾病发病机理，也为开发针对性治疗药物如抗炎、抗肿瘤药物提供理论基础。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于基础医学研究中探讨炎症相关疾病机制、评估药物干预对TNFAIP3表达的影响，或解析其在肿瘤微环境中的调控作用，为科研人员开展免疫调节、疾病标志物筛选等课题提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A20 ELISA Kit; AISBL ELISA Kit; MGC104522 ELISA Kit; MGC138687 ELISA Kit; MGC138688 ELISA Kit; OTU domain containing protein 7C ELISA Kit; OTU domain-containing protein 7C ELISA Kit; OTUD7C ELISA Kit; Putative DNA binding protein A20 ELISA Kit; Putative DNA-binding protein A20 ELISA Kit; TNAP3_HUMAN ELISA Kit; TNF alpha-induced protein 3 ELISA Kit; TNFA1P2 ELISA Kit; TNFAIP 3 ELISA Kit; TNFAIP3 (A20) ELISA Kit; TNFAIP3 ELISA Kit; Tumor necrosis factor alpha induced protein 3 ELISA Kit; Tumor necrosis factor alpha-induced protein 3 ELISA Kit; Tumor necrosis factor induced protein 3 ELISA Kit; Tumor necrosis factor inducible protein A20 ELISA Kit; tumor necrosis factor, alpha-induced protein 3 ELISA Kit; Zinc finger protein A20 ELISA Kit
缩写：TNFAIP3
Uniprot No.：P21580
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TNFAIP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 82-92 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 94-106 | |||||
回收率：
| The recovery of human TNFAIP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-94 | ||||
| EDTA plasma (n=4) | 99 | 95-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.4962.4582.4772.3447501.6581.7881.7231.5903751.0851.1951.1401.007187.50.6900.6850.6880.555940.4540.4670.4610.328470.3200.3210.3210.18823.50.2310.2450.2380.10500.1350.1310.133 | ||||||
货期：3-5 working days
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