首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子肿瘤坏死因子α(TNF-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TNF含量。TNF（肿瘤坏死因子）是一种重要的细胞因子，在免疫调节、炎症反应等生理病理过程中发挥关键作用。研究表明，TNF可激活多条信号通路，介导细胞存活、凋亡、炎症因子释放等。异常的TNF表达与多种疾病如类风湿关节炎等密切相关，是药物研发热门靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，本试剂盒适用于兔模型在病原体感染、炎症机制解析、药物干预效果评估等基础科研领域的研究需求，为探索TNF-α在免疫应答中的动态变化提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
缩写：TNF
Uniprot No.：P04924
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TNFα in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit TNFα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 87-99 | ||||
| EDTA plasma (n=4) | 92 | 87-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 50002.9052.8992.9022.789 25002.3662.3562.3612.248 12501.6391.6041.6221.509 6250.9710.9390.9550.842 3120.4880.4560.4720.359 1560.2750.2590.2670.154 780.1790.1710.1750.062 00.1160.1100.113 | ||||||
货期：3-5 working days
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