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Mouse Tumor necrosis factor α,TNF-α ELISA KIT

  • 中文名： 小鼠肿瘤坏死因子α(TNF-α)ELISA KIT
货号: IPD-E04741m
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠肿瘤坏死因子α(TNF-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TNF含量。TNF（肿瘤坏死因子）是一种多功能细胞因子，在炎症、免疫调节等生理和病理过程中发挥关键作用。研究机制方面，TNF 与其受体结合，激活多条信号通路，引发细胞增殖、凋亡等反应，其异常表达与多种疾病如肿瘤、自身免疫病密切相关。试剂盒检测范围为15.6 pg/ml-1000 pg/ml，适用于体外实验中炎症相关动物模型的血清/血浆样本分析、免疫细胞激活后的细胞因子分泌检测，以及疾病模型组织内TNF-α表达水平的动态监测。为炎症机制研究、药物筛选评价及免疫调控实验提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
  • 缩写：
    TNF
  • Uniprot No.：
    P06804
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 pg/ml-1000 pg/ml
  • 灵敏度：
    3.9 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    		SampleSerum(n=4)
    1:1Average %86

    		Range %80-92
    1:2Average %95

    		Range %91-105
    1:4Average %100

    		Range %92-110
    1:8Average %92

    		Range %86-98
  • 回收率：
    The recovery of mouse TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange
    Serum (n=5)  9389-98
    EDTA plasma (n=4)9590-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.7932.8222.8082.651
    5001.8891.9431.9161.759
    2501.2451.2111.2281.071
    1250.7290.6860.7080.551
    62.50.3730.4040.3880.232
    31.20.2870.2980.2920.136
    15.60.2310.2440.2380.081
    00.1520.1610.157

  • 本试剂盒所含材料：


    • An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.

    • Two bottles of lyophilized standard --- Reconstitute one bottle of standard with sample diluent at dilution series and draw the standard curve.

    • Biotin-labeled TNF-α antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.

    • HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.

    • Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.

    • HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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