Rabbit Monoclonal CDK4 Antibody

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     Western blot analysis of Cdk4 in K562, C6, 3T3, Hela lysates using CDK4 antibody.    

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     Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cdk4 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

CDK4 (Cyclin-Dependent Kinase 4) is a serine/threonine kinase critical for regulating the cell cycle, particularly during the G1 phase. It partners with cyclin D to form a complex that phosphorylates retinoblastoma (Rb) protein, facilitating the transition from G1 to S phase and promoting cell proliferation. Dysregulation of CDK4 is implicated in various cancers, where overexpression, gene amplification, or mutations can lead to uncontrolled cell division. For instance, CDK4/6 inhibitors like palbociclib are clinically approved for hormone receptor-positive breast cancer, underscoring its therapeutic relevance.

CDK4 antibodies are essential tools in biomedical research, enabling the detection and quantification of CDK4 expression in cells and tissues. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and flow cytometry to study CDK4’s role in cancer progression, drug resistance, and cell cycle mechanisms. These antibodies also aid in evaluating the efficacy of CDK4-targeted therapies and identifying biomarkers for patient stratification. In clinical diagnostics, CDK4 immunohistochemical staining helps characterize tumors, such as distinguishing melanoma from benign lesions or assessing liposarcoma subtypes.

Overall, CDK4 antibodies bridge basic research and clinical applications, offering insights into oncogenic pathways and supporting the development of precision oncology strategies. Their utility in both experimental and diagnostic settings highlights CDK4’s significance as a molecular target in cancer biology.