| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC42; CDC42Hs; G25K; TKS |
| Entrez GeneID | 998 |
| WB Predicted band size | Calculated MW: 21 kDa; Observed MW: 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Recombinant protein of human CDC42 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3篇与CDC42抗体相关的文献摘要信息，涵盖不同研究场景中的抗体应用：
---
1. **文献名称**: *CDC42 regulates apical junction formation in human bronchial epithelial cells through PAK and myosin activation*
**作者**: Rodriguez-Boulan E, et al.
**摘要**: 本研究使用CDC42特异性抗体(克隆号：H-102)进行免疫荧光染色，揭示CDC42通过激活PAK和肌球蛋白调节人气道上皮细胞的顶端连接形成。抗体验证通过siRNA敲降及阴性对照实验确认特异性。
2. **文献名称**: *Distinct roles of CDC42 in tubulogenesis and cell polarization during kidney development*
**作者**: Zegers MMP, et al.
**摘要**: 通过Western blot(抗体品牌：Santa Cruz, sc-87)和免疫组织化学分析，发现CDC42在小鼠肾脏发育中调控肾小管形成和细胞极性。研究强调该抗体在区分CDC42异构体中的有效性。
3. **文献名称**: *CDC42-mediated phagocytosis requires spatial coordination of membrane protrusions via ARP2/3 complex*
**作者**: Swanson JA, et al.
**摘要**: 利用单克隆抗体(Anti-CDC42 clone 44H4)抑制巨噬细胞CDC42活性，结合活细胞成像技术，证明CDC42通过调控ARP2/3复合体介导吞噬作用的膜突起空间协调。
---
以上文献均聚焦CDC42抗体在细胞生物学研究中的应用，涵盖蛋白定位、功能验证及信号机制解析。建议通过PubMed或期刊官网输入标题查询全文。
CDC42. a member of the Rho family of small GTPases, plays a critical role in regulating diverse cellular processes, including cell polarity, cytoskeletal reorganization, vesicle trafficking, and cell cycle progression. It functions as a molecular switch, cycling between an active GTP-bound state and an inactive GDP-bound state, thereby mediating signal transduction pathways that influence cell migration, proliferation, and differentiation. CDC42 dysregulation has been implicated in various diseases, such as cancer, neurological disorders, and immune dysfunction.
Antibodies targeting CDC42 are essential tools for studying its expression, localization, and activity in biological systems. These antibodies are widely used in techniques like Western blotting, immunofluorescence, flow cytometry, and immunoprecipitation to detect CDC42 protein levels, assess activation status (e.g., using conformation-specific antibodies for GTP-bound CDC42), and investigate its interaction partners. Researchers often validate CDC42 antibodies for specificity using knockout cell lines or siRNA-mediated knockdown to minimize cross-reactivity with related GTPases like Rac or RhoA.
Applications of CDC42 antibodies extend to both basic research and translational studies, including understanding cancer metastasis, neuronal development, and immune cell signaling. Commercial CDC42 antibodies are available as monoclonal or polyclonal variants, with selection depending on experimental requirements. Proper controls and optimization remain crucial for reliable data interpretation, given the dynamic regulation and context-dependent roles of CDC42 in cellular physiology.
×
