首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肿瘤坏死因子α(TNF-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的TNF含量。TNF即肿瘤坏死因子，是一种参与炎症反应与免疫调节的细胞因子。其背景在于它与多种疾病如类风湿关节炎、肿瘤等相关。研究机制主要围绕其介导细胞信号传导通路，能激活免疫细胞、诱导炎症因子表达，还可影响细胞的存活、增殖与凋亡。试剂盒检测范围为7.8 pg/mL-500 pg/mL，该产品广泛应用于基础科研领域，包括炎症信号通路研究、药物作用靶点验证、疾病动物模型评估及体外细胞因子分泌水平检测等场景，为生命科学研究和生物医药开发提供可靠的工具支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：APC1 ELISA Kit; APC1 protein ELISA Kit; Cachectin ELISA Kit; DIF ELISA Kit; Differentiation inducing factor ELISA Kit; Macrophage cytotoxic factor ELISA Kit; Tnf ELISA Kit; TNF superfamily member 2 ELISA Kit; TNF superfamily, member 2 ELISA Kit; TNF, macrophage derived ELISA Kit; TNF, monocyte derived ELISA Kit; TNF-a ELISA Kit; TNF-alpha ELISA Kit; TNFA ELISA Kit; TNFA_HUMAN ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor (TNF superfamily member 2) ELISA Kit; Tumor necrosis factor alpha ELISA Kit; Tumor necrosis factor ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; Tumor Necrosis Factor, Membrane Form ELISA Kit; Tumor necrosis factor, soluble form ELISA Kit
缩写：TNF
Uniprot No.：P01375
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 89-99 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 88-102 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 85-100 | |||||
回收率：
| The recovery of human TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 94-101 | ||||
| EDTA plasma (n=4) | 96 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 5002.6072.7052.6562.482 2502.2962.3072.3022.128 1251.6421.6241.6331.459 62.50.9120.9400.9260.752 31.20.4890.5040.4970.323 15.60.3170.3280.3230.149 7.80.2820.2710.2770.103 00.1720.1750.174 | ||||||
货期：3-5 working days
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