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产品描述：马肿瘤坏死因子α(TNF-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNF含量。TNF（肿瘤坏死因子）是一种多功能促炎细胞因子，主要由巨噬细胞分泌，与肿瘤坏死因子受体结合发挥功能。其在细胞凋亡、炎症和免疫反应中起重要作用，与多种疾病如肿瘤、炎症和自身免疫性疾病相关。研究TNF机制有助于开发针对相关疾病的治疗策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研场景下对马属动物炎症相关模型的机制研究、药物干预效果评价或基础免疫学分析，例如通过检测血清或组织中的TNF-α浓度动态，揭示炎症相关疾病的分子调控网络。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
缩写：TNF
Uniprot No.：P29553
种属：Equus caballus (Horse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of horse TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 84-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of horse TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 83-98 | ||||
| EDTA plasma (n=4) | 94 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6012.5482.5752.4095002.2482.2112.2302.0642501.6311.6161.6241.4581250.9050.9250.9150.74962.50.4750.4830.4790.31331.20.3120.3210.3170.15115.60.2550.2680.2620.09600.1640.1670.166 | ||||||
货期：3-5 working days
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