首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠toll样受体4(TLR4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TLR4含量。TLR4即Toll样受体4，是天然免疫系统中的重要模式识别受体。它可识别病原体相关分子模式启动天然免疫应答。研究机制方面，其与配体结合后触发信号级联反应，激活转录因子，调控免疫相关基因表达，在抗感染、炎症反应等方面有重要作用。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研领域中对TLR4蛋白水平的动态监测，例如炎症反应机制研究、感染模型评估、免疫调节药物开发等实验场景，96孔板设计可满足高通量样本分析需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tlr4; Toll-like receptor 4; Toll4; CD antigen CD284
缩写：TLR4
Uniprot No.：Q9QX05
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TLR4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-96 | |||||
回收率：
| The recovery of rat TLR4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-101 | ||||
| EDTA plasma (n=4) | 94 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.0341.9341.9841.820201.2941.2091.2521.088100.8030.7930.7980.63450.5580.5480.5530.3892.50.3950.3820.3890.2251.250.3140.3040.3090.1450.6250.2420.2380.2400.07600.1640.1630.164 | ||||||
货期：3-5 working days
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