首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠可溶性Toll样受体4(sTLR4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TLR4含量。TLR4（Toll样受体 4）是一种模式识别受体，在先天性免疫中发挥关键作用。它能识别病原体相关分子模式，激活下游信号通路，触发炎症反应。对其研究机制有助于了解免疫性疾病、炎症性疾病的发病机理，为相关疾病治疗提供潜在靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于炎症机制研究、免疫相关疾病动物模型（如败血症、非酒精性脂肪肝）的分子标志物分析，以及药物干预实验中 sTLR4 动态变化的监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tlr4 ELISA Kit; Lps ELISA Kit; Toll-like receptor 4 ELISA Kit; EC 3.2.2.6 ELISA Kit; CD antigen CD284 ELISA Kit
缩写：TLR4
Uniprot No.：Q9QUK6
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse sTLR4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 95 | ||||
| Range % | 88-98 | |||||
| 1:10 | Average % | 100 | ||||
| Range % | 96-103 | |||||
| 1:20 | Average % | 94 | ||||
| Range % | 88-97 | |||||
| 1:40 | Average % | 99 | ||||
| Range % | 95-102 | |||||
回收率：
| The recovery of mouse sTLR4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-95 | ||||
| EDTA plasma (n=4) | 102 | 97-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.7582.7132.7362.619 52.0122.0872.0501.933 2.51.2041.1781.1911.074 1.250.6630.6520.6580.541 0.6250.3780.3660.3720.255 0.3120.2750.2810.2780.161 0.1560.1940.1870.1910.074 00.1160.1180.117 | ||||||
货期：3-5 working days
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