首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：犬血栓调节蛋白(TM)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的THBD含量。THBD即凝血酶调节蛋白，它是血管内皮细胞表面的凝血酶受体。其背景上与凝血、炎症等密切相关。研究机制方面，它能和凝血酶结合，改变其底物特异性，激活蛋白C，进而发挥抗凝、抗炎、保护血管内皮等作用，在多种疾病研究中有重要意义。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，是研究犬类凝血功能异常、心血管疾病及炎症相关机制的重要生物标志物；适用于科研领域中对犬类血栓形成机制、内皮功能障碍及药物干预效果的体外研究，为动物模型实验提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：THBD ELISA Kit; Thrombomodulin ELISA Kit; TM ELISA Kit; CD antigen CD141 ELISA Kit
缩写：THBD
Uniprot No.：Q5W7P8
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:20 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 88-94 | |||||
| 1:80 | Average % | 93 | ||||
| Range % | 89-97 | |||||
回收率：
| The recovery of dog TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 83-94 | ||||
| EDTA plasma (n=4) | 93 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average15000.0870.0860.0877500.1480.1420.1453750.2340.2290.232187.50.3470.3380.34393.750.5620.5760.56946.880.8650.9210.89323.441.4641.3921.42802.6772.5682.623 | ||||||
货期：3-5 working days
×
