| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BLNK; BASH; SLP65; B-cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Cytoplasmic adapter protein; Src homology 2 domain-containing leukocyte protein of 65 kDa |
| Entrez GeneID | 29760 |
| WB Predicted band size | Calculated MW: 50 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human BLNK |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于BLNK抗体的3篇代表性文献的简要总结：
1. **文献名称**：*BLNK: A Central Linker Protein in B Cell Activation*
**作者**：Fu, C., Turck, C.W., Kurosaki, T., Chan, A.C.
**摘要**：该研究首次克隆并鉴定了BLNK蛋白，揭示了其在B细胞受体(BCR)信号传导中的关键作用。BLNK通过募集Syk激酶和PLCγ，促进下游钙离子信号通路和NF-κB激活，是B细胞发育和功能的核心衔接分子。
2. **文献名称**：*Defective B Cell Development and Function in BLNK-Deficient Mice*
**作者**：Minegishi, Y., Rohrer, J., Coustan-Smith, E., et al.
**摘要**：通过BLNK基因敲除小鼠模型，研究发现BLNK缺失导致B细胞发育阻滞在前B细胞阶段，并显著削弱BCR介导的增殖与抗体分泌，证实BLNK对B细胞成熟及适应性免疫不可或缺。
3. **文献名称**：*Human BLNK Deficiency Associated with Impaired Antibody Response and Recurrent Infections*
**作者**：Conley, M.E., Dobbs, A.K., Farmer, D.M., et al.
**摘要**：报道了人类BLNK基因突变导致的免疫缺陷病例，患者表现为B细胞数量减少、抗体应答低下及反复感染，进一步验证BLNK在人类体液免疫中的关键作用，并提示其作为临床诊断标记物的潜力。
这些文献涵盖了BLNK的基础机制、动物模型及临床相关性研究，均涉及BLNK抗体的应用(如Western blot、流式检测)以解析其功能。
BLNK (B-cell linker protein), also known as SLP-65 or BCAP, is a critical adaptor protein in B-cell receptor (BCR) signaling. Expressed predominantly in B lymphocytes, BLNK acts as a scaffold to coordinate downstream signaling molecules upon BCR activation. It facilitates the assembly of key signaling complexes by recruiting proteins like PLCγ, BTK, and VAV through phosphorylated tyrosine residues, enabling calcium mobilization, MAPK activation, and transcriptional regulation. These processes are essential for B-cell development, proliferation, and differentiation into antibody-producing plasma cells.
Defects in BLNK expression or function are linked to immunodeficiency disorders. For example, BLNK mutations cause agammaglobulinemia, characterized by impaired B-cell maturation and reduced antibody production. BLNK antibodies are vital tools for studying BCR signaling dynamics, detecting protein expression in research or diagnostic settings (e.g., evaluating B-cell malignancies), and exploring therapeutic targets. In cancers like pre-B cell acute lymphoblastic leukemia (ALL), aberrant BLNK expression may correlate with disease progression, making it a potential biomarker. Researchers commonly use BLNK-specific antibodies in techniques such as Western blotting, flow cytometry, and immunohistochemistry to dissect B-cell signaling pathways or assess immune pathologies. Their applications extend to preclinical studies targeting BLNK-associated pathways to modulate immune responses or treat B-cell disorders.
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