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产品描述：人转化生长因子β3(TGF-β3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的TGFB3含量。TGFB3即转化生长因子β3，在胚胎发育、组织修复等生理过程中发挥重要作用。其研究机制方面，主要通过与受体结合，激活下游信号通路，调控细胞增殖、分化、迁移，对多种生物学过程产生广泛影响，在医学研究中具有潜在价值。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品适用于体外培养细胞模型中细胞因子分泌研究、疾病相关分子机制探索，以及动物实验样本中TGF-β3的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ARVD ELISA Kit; ARVD1 ELISA Kit; FLJ16571 ELISA Kit; LDS5 ELISA Kit; MGC105479 ELISA Kit; MGC118722 ELISA Kit; prepro-transforming growth factor beta-3 ELISA Kit; RNHF ELISA Kit; TGF beta 3 ELISA Kit; TGF beta3 ELISA Kit; TGF-beta-3 ELISA Kit; TGFB 3 ELISA Kit; Tgfb3 ELISA Kit; TGFB3_HUMAN ELISA Kit; transforming growth factor beta 3 ELISA Kit; Transforming growth factor beta-3 ELISA Kit
缩写：TGFB3
Uniprot No.：P10600
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-β3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 90-104 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-91 | |||||
回收率：
| The recovery of human TGF-β3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-98 | ||||
| EDTA plasma (n=4) | 98 | 96-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.6992.7522.7262.571 5002.4352.3992.4172.262 2501.8081.8751.8421.687 1251.0651.0551.0600.905 62.50.6780.7350.7070.552 31.20.4010.4220.4120.257 15.60.2670.2750.2710.116 00.1490.1600.155 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TGFB3 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled TGFB3 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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