首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠转化生长因子β2(TGF-β2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TGFB2含量。TGFB2属于转化生长因子β超家族成员，在胚胎发育、组织修复、免疫调节等生理过程中发挥关键作用。研究机制方面，它主要通过与相应受体结合，激活下游信号通路，影响细胞增殖、分化和迁移，其异常表达与多种疾病如肿瘤、纤维化等相关。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，可用于探索炎症反应、器官纤维化、胚胎发育或肿瘤发生等领域的分子机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tgfb2 ELISA Kit; Transforming growth factor beta-2 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-2 ELISA Kit; TGF-beta-2)] ELISA Kit
缩写：TGFB2
Uniprot No.：Q07257
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TGF-β2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of rat TGF-β2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 83 | 81-85 | ||||
| EDTA plasma (n=4) | 96 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.8692.8972.8832.691 5002.2312.2672.2492.057 2501.5441.5981.5711.379 1250.9690.9870.9780.786 62.50.6380.6090.6240.432 31.20.4520.4720.4620.270 15.60.3070.3210.3140.122 00.1910.1930.192 | ||||||
货期：3-5 working days
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