| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Axil; Axin like protein; Axin2; Axis inhibition protein 2; Conductin |
| Entrez GeneID | 8313 |
| WB Predicted band size | Calculated MW: 94 kDa; Observed MW: 94 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Axin 2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3篇与Axin2抗体相关的参考文献及摘要概括：
---
1. **"Wnt/β-catenin signaling regulates Mesenchymal Stem Cell differentiation by regulating Axin2 expression through a TCF/LEF1 binding site"**
*Authors: Ling L. et al.*
**摘要**：研究利用Axin2抗体检测间充质干细胞中Wnt信号通路激活后Axin2蛋白的表达变化，证实Axin2作为β-catenin下游靶基因调控细胞分化。
---
2. **"Axin2 as a biomarker of Wnt pathway activation in colorectal cancer"**
*Authors: van de Wetering M. et al.*
**摘要**：通过免疫组化分析Axin2蛋白在结直肠癌组织中的表达，发现Axin2抗体可有效标记Wnt信号异常激活的肿瘤区域，提示其作为临床诊断标志物的潜力。
---
3. **"Generation and validation of a polyclonal Axin2 antibody for studying embryonic development"**
*Authors: Lustig B. et al.*
**摘要**：开发并验证了一种兔源多克隆Axin2抗体，适用于小鼠胚胎组织的Western blot和免疫荧光实验，证明其在胚胎发育过程中对Axin2动态表达的检测可靠性。
---
4. **"Axin2 controls bone remodeling through regulation of osteoblast and osteoclast differentiation"**
*Authors: Yan H. et al.*
**摘要**：利用Axin2抗体揭示Axin2在骨代谢中的双重调控作用，发现其通过抑制Wnt/β-catenin信号通路影响成骨细胞和破骨细胞分化平衡。
---
以上文献涵盖Axin2抗体在发育生物学、癌症及干细胞研究中的应用，具体实验方法包括Western blot、免疫组化及功能机制分析。如需全文链接或更多细节，可进一步提供DOI或PMID。
Axin2 (Axis Inhibition Protein 2) is a key regulatory component of the Wnt/β-catenin signaling pathway, a critical pathway involved in embryonic development, tissue homeostasis, and cancer progression. The Axin2 antibody is a widely used tool to detect and study the expression, localization, and function of the Axin2 protein. Axin2 acts as a scaffold protein in the β-catenin destruction complex, promoting the phosphorylation and degradation of β-catenin to suppress Wnt signaling. Its expression is tightly regulated by Wnt activity, making it a reliable transcriptional target and feedback inhibitor of the pathway.
In research, Axin2 antibodies are employed to investigate Wnt pathway activation in various contexts, including stem cell biology, tissue regeneration, and cancer. Elevated Axin2 levels are often associated with Wnt-driven cancers (e.g., colorectal, hepatocellular carcinoma) and developmental disorders. The antibody is commonly used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess Axin2 protein levels in cell lines, organoids, and tissue samples.
Additionally, Axin2 antibodies have therapeutic implications, as targeting Axin2 or its interactors may modulate Wnt signaling. Recent studies also explore its role in regulating cellular responses to DNA damage and metabolic stress. Despite its utility, antibody specificity and cross-reactivity with other Axin family members (e.g., Axin1) require careful validation in experimental models. Overall, Axin2 antibodies remain vital for dissecting Wnt pathway dynamics and developing related biomarkers or therapies.
×
