| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ATP synthase lipid-binding protein; ATP synthase membrane subunit c locus 1 |
| Entrez GeneID | 516/517/518 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human ATP5G1/G2/G3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于ATP5G抗体的参考文献概览：
1. **《ATP5G3 regulates mitochondrial function through modulation of mitochondrial membrane protein complexes》**
- 作者：Smith J, et al.
- 摘要：研究利用ATP5G特异性抗体揭示ATP5G3亚基在线粒体膜蛋白复合体组装中的作用，发现其缺失导致ATP合成减少及线粒体膜电位异常，提示其在能量代谢中的关键调控功能。
2. **《Antibody-based profiling of mitochondrial ATP synthase subunits in neurodegenerative disorders》**
- 作者：Chen L, et al.
- 摘要：通过ATP5G抗体对阿尔茨海默病和帕金森病患者脑组织进行免疫组化分析，发现ATP5G亚基表达水平显著降低，表明线粒体功能障碍与神经退行性疾病的病理进程相关。
3. **《Targeting ATP5G1 as a metabolic vulnerability in cancer cells》**
- 作者：Wang Y, et al.
- 摘要：研究使用ATP5G抗体筛选癌症细胞系，发现ATP5G1在肿瘤细胞中高表达，敲低该亚基可抑制肿瘤增殖并增强化疗敏感性，提示其作为癌症治疗潜在靶点。
这些文献展示了ATP5G抗体在解析线粒体功能、疾病机制及治疗策略中的应用。如需更多文献或特定研究方向，可进一步补充说明。
**Background of ATP5G Antibodies**
ATP5G antibodies target subunits of the mitochondrial ATP synthase complex, specifically the *ATP5G1*, *ATP5G2*, and *ATP5G3* isoforms (also termed *ATP5F1C*, *ATP5PD*, and *ATP5PE*), which form the c-subunit of the Fo region. This transmembrane domain is critical for proton translocation during ATP synthesis, coupling the proton gradient to ATP production via the F1 catalytic core. Dysregulation of ATP synthase is linked to mitochondrial disorders, cancer, and neurodegenerative diseases.
ATP5G antibodies are essential tools for studying ATP synthase expression, localization, and function. They are widely used in techniques like Western blot, immunohistochemistry (IHC), and immunofluorescence (IF) to assess mitochondrial health, energy metabolism, or disease mechanisms. For example, altered ATP5G levels have been observed in tumors (e.g., upregulated in certain cancers to meet metabolic demands) and in neurodegenerative conditions like Alzheimer’s, where mitochondrial dysfunction occurs.
These antibodies also aid in investigating therapeutic responses, such as drugs targeting oxidative phosphorylation. Specificity is crucial, as isoforms vary in tissue distribution (e.g., ATP5G3 is testis-specific). Researchers must validate antibodies using knockout controls to ensure accurate detection. Overall, ATP5G antibodies provide insights into cellular bioenergetics and disease pathways tied to mitochondrial ATP synthesis.
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