Rabbit Monoclonal ATP6V0D1 Antibody

The ATP6V0D1 antibody is a tool used to detect the ATP6V0D1 protein, a subunit of the vacuolar-type H+-translocating ATPase (V-ATPase) complex. V-ATPases are proton pumps critical for acidifying intracellular organelles like lysosomes, endosomes, and secretory vesicles, thereby maintaining pH gradients essential for processes such as protein degradation, membrane trafficking, and synaptic transmission. The ATP6V0D1 subunit, part of the V0 domain, plays a structural role in V-ATPase assembly and proton translocation. Research on ATP6V0D1 focuses on its involvement in cellular homeostasis, autophagy, and diseases like cancer, neurodegenerative disorders, and osteoporosis. In cancer, V-ATPases are linked to tumor metastasis and drug resistance, with ATP6V0D1 overexpression observed in certain malignancies. In neurodegeneration, dysfunctional lysosomal acidification due to V-ATPase defects contributes to pathologies like Alzheimer’s and Parkinson’s diseases. ATP6V0D1 antibodies are utilized in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to study protein expression, localization, and regulation across tissues and disease models. These antibodies are crucial for elucidating ATP6V0D1’s role in cellular physiology and its potential as a therapeutic target. Validation of antibody specificity, often via knockout controls or peptide blocking, ensures accurate detection, minimizing cross-reactivity with related V-ATPase subunits.