首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人端粒酶(TE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、尿液、组织匀浆样本中的TE含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研人员可通过分析疾病模型动物或体外培养细胞中的端粒酶表达动态，探索肿瘤发生机制、评估抗衰老药物对端粒酶活性的调控作用，或利用尿液样本开展无创性研究。其宽泛的样本兼容性为组织水平研究、细胞实验数据验证及生物标志物筛选提供了可靠工具，适用于基础医学、药理学及分子生物学领域的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：TE
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, urine, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-96 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 92-98 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human TE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 80-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.6302.5012.5662.464102.1612.0622.1122.01051.6021.5411.5721.4702.50.9310.8980.9150.8131.250.5320.5010.5170.4150.6250.3220.3140.3180.2160.3120.2410.2270.2340.13200.1050.0990.102 | ||||||
货期：3-5 working days
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