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产品描述：人酪氨酰DNA 磷酸二酯酶1（TDP1）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TDP1含量。TDP1是一种具有重要生物学意义的靶点。其背景在于在DNA修复过程中发挥作用。研究机制方面，它能特异性地去除3'-磷酸酪氨酸，修复由拓扑异构酶I介导产生的DNA单链断裂，对维护基因组的稳定性至关重要，是潜在的抗癌药物作用靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品适用于科研领域探索TDP1在神经退行性疾病、肿瘤耐药机制中的表达调控，或评估化疗药物对DNA修复通路的影响，为分子生物学及疾病机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AI838772 ELISA Kit; AW493413 ELISA Kit; FLJ11090 ELISA Kit; MGC104252 ELISA Kit; MGC112732 ELISA Kit; RP24-311F12.2 ELISA Kit; SCAN1 ELISA Kit; TDP1 ELISA Kit; TYDP ELISA Kit; TYDP1_HUMAN ELISA Kit; Tyr-DNA phosphodiesterase 1 ELISA Kit; Tyrosyl-DNA phosphodiesterase 1 ELISA Kit
缩写：TDP1
Uniprot No.：Q9NUW8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TDP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 105 | ||||
| Range % | 100-112 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-94 | |||||
回收率：
| The recovery of human TDP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 91 | 86-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40002.4182.5202.4692.314 20001.9601.9761.9681.813 10001.4431.5041.4741.319 5000.8840.9390.9120.757 2500.5930.6340.6140.459 1250.4260.4490.4380.283 62.50.3120.3090.3110.156 00.1580.1520.155 | ||||||
货期：3-5 working days
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