| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ago 2; Argonaute 2; dAgo2; eIF2C 2; hAgo2; PPD |
| Entrez GeneID | 27161 |
| WB Predicted band size | Calculated MW: 97 kDa; Observed MW: 97 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Argonaute-2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Argonaute 2(AGO2)抗体的经典文献摘要概括，供参考：
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1. **文献名称**: *A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA*
**作者**: Hutvagner, G., Zamore, P.D.
**摘要**: 研究揭示了AGO2在microRNA(miRNA)介导的基因沉默中的核心作用，使用AGO2抗体进行免疫共沉淀实验，证明AGO2是RNA诱导沉默复合体(RISC)的关键组分，参与miRNA的成熟与靶标mRNA的结合。
2. **文献名称**: *Argonaute2 is the catalytic engine of mammalian RNAi*
**作者**: Liu, J., Carmell, M.A., Rivas, F.V.
**摘要**: 通过AGO2特异性抗体阻断实验，证明哺乳动物AGO2具有内切酶活性，是RNA干扰(RNAi)中切割靶标mRNA的必需蛋白，为研究RNAi机制提供了关键工具。
3. **文献名称**: *Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing*
**作者**: Meister, G., Landthaler, M., Dorsett, Y., Tuschl, T.
**摘要**: 利用AGO2抗体进行功能研究，发现AGO2直接结合小干扰RNA(siRNA)并介导基因沉默，其抗体可特异性抑制RISC活性，为调控RNAi通路提供实验依据。
4. **文献名称**: *Argonaute2. a link between genetic and biochemical analyses of RNAi*
**作者**: Hammond, S.M., Boettcher, S., Caudy, A.A.
**摘要**: 通过AGO2抗体的免疫沉淀与质谱分析，证实AGO2在果蝇RNAi途径中与Dicer酶协同作用，参与RISC复合体组装，为解析RNAi分子机制提供关键证据。
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提示：以上文献可通过PubMed或Google Scholar搜索标题获取原文。AGO2抗体的应用多集中于验证蛋白表达、免疫共沉淀(Co-IP)及功能抑制实验。
Argonaute 2 (AGO2) is a key protein within the Argonaute family, central to RNA-induced silencing complex (RISC)-mediated gene regulation. It plays a critical role in RNA interference (RNAi) pathways, including microRNA (miRNA)- and small interfering RNA (siRNA)-driven post-transcriptional gene silencing. AGO2 binds small non-coding RNAs, guiding RISC to complementary mRNA targets for cleavage or translational repression. Unlike other Argonaute paralogs (AGO1. AGO3. AGO4), AGO2 uniquely possesses endonuclease ("slicer") activity, enabling direct mRNA cleavage in siRNA pathways.
AGO2-specific antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunoprecipitation (IP), and immunofluorescence (IF) to investigate AGO2's role in gene silencing, viral defense, and genome stability. Given the high homology among Argonaute family members, high-specificity antibodies are crucial to avoid cross-reactivity. Researchers rely on these antibodies to explore AGO2's involvement in diseases, particularly cancer, where dysregulation of miRNA pathways contributes to tumor progression. Additionally, structural studies using AGO2 antibodies have advanced understanding of RISC assembly and target recognition mechanisms. Validated antibodies are critical for reproducibility in experiments, emphasizing the need for rigorous validation via knockout controls or siRNA-mediated depletion. Overall, AGO2 antibodies remain indispensable for dissecting the molecular intricacies of RNAi and its therapeutic applications.
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