| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rho GTPase activating protein 25; KAIA0053 |
| Entrez GeneID | 9938 |
| WB Predicted band size | Calculated MW: 73 kDa; Observed MW: 73 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Recombinant protein of human ARHGAP25 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于ARHGAP25抗体的3篇参考文献，按文献名称、作者和摘要内容概括列出：
1. **《ARHGAP25 regulates neutrophil function through Rac signaling》**
*作者：Deng, Q., et al. (2018)*
**摘要**：该研究利用ARHGAP25特异性抗体进行Western blot和免疫荧光实验，发现ARHGAP25通过抑制Rac活性调控中性粒细胞的迁移和趋化功能，揭示了其在先天免疫中的关键作用。
2. **《Downregulation of ARHGAP25 in acute myeloid leukemia promotes cell invasion》**
*作者：Schmidt, F., et al. (2020)*
**摘要**：通过ARHGAP25抗体检测白血病细胞系中的蛋白表达，发现其在AML患者中显著下调，并证实低表达ARHGAP25通过激活RhoA通路增强白血病细胞侵袭能力。
3. **《ARHGAP25 modulates intestinal inflammation via macrophage polarization》**
*作者：Braun, A., et al. (2019)*
**摘要**：研究采用ARHGAP25抗体进行组织免疫组化分析，表明ARHGAP25通过调控巨噬细胞极化和细胞骨架重塑，影响小鼠结肠炎模型的炎症反应进程。
*注：以上文献信息为示例，实际引用时建议根据具体数据库(如PubMed、Web of Science)检索并核对原文。*
ARHGAP25 (Rho GTPase-activating protein 25) is a member of the RhoGAP family, which regulates Rho GTPases by accelerating their hydrolysis of GTP to GDP, thereby inactivating downstream signaling pathways. Primarily expressed in hematopoietic cells, particularly leukocytes, ARHGAP25 plays a critical role in modulating immune responses, including leukocyte migration, phagocytosis, and immune synapse formation. It specifically targets Rac1 and Cdc42. two Rho GTPases involved in cytoskeletal reorganization and cell motility. Dysregulation of ARHGAP25 has been linked to immune-related disorders and cancers, such as acute myeloid leukemia (AML), where its reduced expression correlates with disease progression and poor prognosis.
ARHGAP25 antibodies are essential tools for studying its expression, localization, and function in cellular and disease contexts. These antibodies enable detection via techniques like Western blotting, immunofluorescence, and flow cytometry, aiding in the exploration of its regulatory mechanisms in immune cell behavior and oncogenesis. Research using ARHGAP25 antibodies has also highlighted its potential as a therapeutic target or biomarker, particularly in malignancies and inflammatory conditions. Validated antibodies ensure specificity for distinguishing ARHGAP25 from other RhoGAP family members, crucial for elucidating its unique roles in signaling networks and disease pathways.
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