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产品描述：兔末端补体复合物C5b-9(TCC C5b-9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TCC C5b-9含量。试剂盒检测范围为7.8 ng/mL-500 ng/mL，该产品适用于基础医学研究中补体系统功能评估、炎症反应机制探索以及自身免疫性疾病模型的建立，例如在肾脏疾病、动脉粥样硬化或神经退行性疾病等领域的实验设计中，可通过检测血清或血浆中的C5b - 9水平，揭示补体终末通路激活程度及其病理学关联。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TCC C5b-9
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 89-109 | ||||
| EDTA plasma (n=4) | 97 | 92-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected5002.6592.5632.6112.5192502.2402.1282.1842.0921251.4881.3751.4321.34062.50.8450.8170.8310.73931.20.4440.4340.4390.34715.60.2470.2390.2430.1517.80.1560.1480.1520.06000.0930.0910.092 | ||||||
货期：3-5 working days
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