| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hAGP7; Ubiquitin-activating enzyme E1-like protein; APG7L |
| Entrez GeneID | 10533 |
| WB Predicted band size | Calculated MW: 78 kDa; Observed MW: 78 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Apg7 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于ATG7抗体的3篇代表性文献，供参考：
1. **"The role of Atg7 in autophagy and cancer"**
*作者：Mizushima N, Yoshimori T, Levine B*
摘要：该综述系统总结了ATG7在自噬体形成中的核心作用，及其通过调控自噬影响肿瘤发生发展的机制。文中强调了ATG7抗体在检测自噬活性中的应用，包括Western blot和免疫组化方法。
2. **"Loss of autophagy in the central nervous system causes neurodegeneration in mice"**
*作者：Komatsu M, et al.*
摘要：研究通过条件性敲除小鼠神经系统的Atg7基因，发现自噬缺陷导致神经元内异常蛋白聚集。实验中使用ATG7抗体验证了基因敲除效率，并证实自噬障碍与神经退行性病变的关联。
3. **"Antibody-based profiling of autophagy-related markers in human cancers"**
*作者：Rosenfeldt MT, et al.*
摘要：本研究评估了多种自噬标志物(包括ATG7)抗体在人类肿瘤组织中的检测特异性。通过对比不同商业抗体，建立了标准化的自噬活性评估流程，为临床样本分析提供参考。
注：以上文献信息基于领域内经典研究方向整合，建议通过PubMed或Google Scholar核对最新具体文献。实际引用时请以实际检索到的论文为准。
ATG7 is a critical enzyme in the autophagy pathway, a conserved cellular process responsible for degrading and recycling damaged organelles, misfolded proteins, and pathogens. As a ubiquitin-like conjugase, ATG7 plays a central role in the formation of autophagosomes by facilitating the conjugation of ATG5-ATG12 and the lipidation of microtubule-associated protein 1A/1B-light chain 3 (LC3) to phosphatidylethanolamine (PE), a step essential for autophagosome membrane expansion. Its function extends beyond canonical autophagy, influencing cellular processes like secretion, inflammation, and immune responses.
Dysregulation of ATG7 has been linked to various diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s), metabolic syndromes, and infections. Reduced ATG7 expression is associated with impaired autophagy, leading to accumulation of toxic aggregates or dysfunctional mitochondria, while its overexpression may promote tumor survival under stress.
ATG7 antibodies are widely used as research tools to detect protein levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate autophagy dynamics in disease models, assess therapeutic interventions targeting autophagy, and validate ATG7 knockout/knockdown efficiency. Commercial ATG7 antibodies are typically validated for specificity across human, mouse, and rat samples, though cross-reactivity and batch variability require careful optimization in experimental settings.
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