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产品描述：猪末端补体复合物C5b-9(TCC C5b-9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TCC C5b-9含量。试剂盒检测范围为18.75 ng/mL-1200 ng/mL，适用于动物模型中的补体激活研究、炎症性疾病机制探索或药物干预效果评估等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TCC C5b-9
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 ng/mL-1200 ng/mL
灵敏度：4.69 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 85-105 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 85-113 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 82-102 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 85-110 | |||||
回收率：
| The recovery of pig TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 88-109 | ||||
| EDTA plasma (n=4) | 97 | 85-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected12002.2172.3192.2682.1306001.7901.7671.7791.6413001.1231.1321.1280.9901500.6750.6510.6630.525750.3970.4030.4000.26237.50.2850.2980.2920.15418.750.2310.2160.2240.08600.1410.1340.138 | ||||||
货期：3-5 working days
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