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产品描述：人TAR DNA结合蛋白43(TARDBP/TDP43)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、脑脊液（CSF）样本中的TARDBP含量。TARDBP基因编码的TDP-43蛋白与DNA和RNA结合，参与RNA转录、剪接和稳定性调节。研究显示，TDP-43蛋白病与神经退行性疾病如肌萎缩侧索硬化症（ALS）和额颞叶痴呆（FTLD）有关，其研究机制涉及TDP-43蛋白在细胞内线粒体损伤和去折叠蛋白反应（UPRmt）的激活。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于神经科学领域基础研究，可用于探索TDP43蛋白在疾病模型中的表达变化、病理机制分析或药物干预实验中的生物标志物检测，例如利用脑脊液样本研究中枢神经系统疾病中TDP43的病理沉积特征，或通过细胞培养上清评估体外模型中蛋白分泌动态。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ALS10 ELISA Kit; OTTHUMP00000002171 ELISA Kit; OTTHUMP00000002172 ELISA Kit; OTTHUMP00000002173 ELISA Kit; TADBP_HUMAN ELISA Kit; TAR DNA binding protein 43 ELISA Kit; TAR DNA binding protein ELISA Kit; TAR DNA-binding protein 43 ELISA Kit; TARDBP ELISA Kit; TDP 43 ELISA Kit; TDP-43 ELISA Kit; TDP43 ELISA Kit
缩写：TARDBP
Uniprot No.：Q13148
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, cerebrospinal fluid (CSF)
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TDP43 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-93 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 90-104 | |||||
回收率：
| The recovery of human TDP43 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 91-104 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.3602.2962.3282.201 101.5681.5701.5691.442 51.0261.0611.0440.917 2.50.6530.6790.6660.539 1.250.4330.4590.4460.319 0.6250.3040.3210.3130.186 0.3120.2190.2230.2210.094 00.1280.1260.127 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TDP43 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled TDP43 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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