首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人突触泡蛋白(SYP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SYP含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，可稳定应用于神经科学基础研究、药物作用机制探索以及脑脊液/外周组织中的突触功能关联分析。科研人员可通过该产品定量评估不同实验条件下（如神经损伤模型、药物干预或基因编辑）SYP的表达水平变化，为探究神经系统疾病病理机制提供可靠工具，适用于体外实验环境下的样本批量检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SYP; Synaptophysin; Major synaptic vesicle protein p38
缩写：SYP
Uniprot No.：P08247
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SYP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 87-98 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human SYP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-100 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.1572.2582.2082.10320001.7761.9241.8501.74510001.4521.5561.5041.3995001.1521.1731.1631.0582500.7510.7650.7580.6531250.4160.4270.4220.31762.50.2620.2680.2650.16000.1040.1050.105 | ||||||
货期：3-5 working days
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