首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人突触融合蛋白-1A(STX1A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆样本中的STX1A含量。STX1A 即 syntaxin 1A，是一种神经元特异性突触蛋白，在神经递质释放中起关键作用。其与多种神经调节蛋白相互作用，参与突触小泡的锚定和融合过程。研究围绕其结构、功能及在神经系统疾病中的异常机制展开，有望为相关疾病治疗提供新方向。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于神经生物学、细胞信号转导等基础研究领域，可用于探索神经退行性疾病机制、突触可塑性调控或药物作用靶点验证等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HPC 1 ELISA Kit; Neuron specific antigen HPC1 ELISA Kit; Neuron-specific antigen HPC-1 ELISA Kit; OTTHUMP00000174615 ELISA Kit; OTTHUMP00000174616 ELISA Kit; OTTHUMP00000174617 ELISA Kit; OTTHUMP00000174618 ELISA Kit; P35-1 ELISA Kit; STX1 ELISA Kit; STX1A ELISA Kit; STX1A_HUMAN ELISA Kit; SYN1A ELISA Kit; Syntaxin 1A (brain) ELISA Kit; Syntaxin 1A brain ELISA Kit; Syntaxin-1A ELISA Kit
缩写：STX1A
Uniprot No.：Q16623
种属：Homo sapiens (Human)
样本类型：serum, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human STX1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human STX1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-104 | ||||
| EDTA plasma (n=4) | 95 | 88-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4892.4522.4712.3345002.0491.9892.0191.8822501.4501.3991.4251.2881250.9130.8670.8900.75362.50.5820.5510.5670.43031.20.3610.3570.3590.22215.60.2460.2340.2400.10300.1360.1380.137 | ||||||
货期：3-5 working days
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