首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠斯钙素-1(STC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的STC1含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，支持多种科研场景应用，包括探究STC1在代谢调控、细胞应激反应中的表达规律，分析疾病模型中STC1的分泌动态，以及评估药物或基因干预对靶蛋白水平的影响本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Stc1 ELISA Kit; Stc ELISA Kit; Stanniocalcin-1 ELISA Kit; STC-1 ELISA Kit
缩写：STC1
Uniprot No.：O55183
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse STC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 87-97 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 95-102 | ||||||
| 1:4 | Average % | 101 | |||||
| Range % | 95-105 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 91-98 | ||||||
回收率：
| The recovery of mouse STC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 87-98 | |||||
| EDTA plasma (n=4) | 99 | 95-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.5582.6332.5962.38720002.2052.2122.2092.00010001.6821.6651.6741.4655000.9900.9950.9930.7842500.4990.5120.5060.2971250.3900.4210.4060.19762.50.2990.3220.3110.10200.2050.2120.209 | |||||||
货期：3-5 working days
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