首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胞外硫酸酯酶Sulf-1(SULF1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SULF1含量。SULF1是一种在细胞外硫酸乙酰肝素内硫酸酯酶，参与调节硫酸乙酰肝素蛋白聚糖的硫酸化。其在肺纤维化和肺腺癌中表达上调，与肿瘤微环境、免疫抑制和肿瘤进展相关，可能成为潜在的治疗靶点。试剂盒检测范围为7.8 pg/mL-500pg/mL，适用于基础科研中探索SULF1在肿瘤生物学、器官发育或退行性疾病中的作用机制，为细胞外基质动态调控、药物靶点筛选等研究提供可靠工具。操作标准化，支持多样本类型并行分析，是研究硫酸酯酶介导的细胞信号网络及病理生理过程的理想选择。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DSulf ELISA Kit; Extracellular sulfatase Sulf 1 ELISA Kit; Extracellular sulfatase Sulf-1 ELISA Kit; Extracellular sulfatase Sulf1 ELISA Kit; HSULF 1 ELISA Kit; HSulf-1 ELISA Kit; Neu5 ELISA Kit; Sulf1 ELISA Kit; SULF1_HUMAN ELISA Kit; Sulfatase FP ELISA Kit; Sulfated ELISA Kit
缩写：SULF1
Uniprot No.：Q8IWU6
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：7.8 pg/mL-500pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SULF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 87-99 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 91-108 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 92-100 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 90-104 | ||||||
回收率：
| The recovery of human SULF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 88-104 | |||||
| EDTA plasma (n=4) | 97 | 89-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5002.4962.4112.4542.3012501.9151.9681.9421.7891251.2841.2371.2611.10862.50.8660.8500.8580.70531.20.4940.5120.5030.35015.60.3490.3650.3570.2047.80.2540.2610.2580.10500.1510.1550.153 | |||||||
货期：3-5 working days
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