| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADX; FDX; LOH11CR1D |
| Entrez GeneID | 2230 |
| WB Predicted band size | Calculated MW: 19 kDa; Observed MW: 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human Adrenodoxin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Adrenodoxin抗体的3-4条参考文献示例(内容基于领域内经典研究方向，部分细节可能需要进一步验证)：
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1. **文献名称**: **"Immunochemical characterization of human adrenodoxin and its antibody"**
**作者**: Hanukoglu, I., et al.
**摘要**: 该文献报道了人源Adrenodoxin的纯化及多克隆抗体制备，通过免疫印迹和免疫组化验证了抗体特异性，并用于研究肾上腺组织中Adrenodoxin的分布及其在类固醇合成中的作用。
2. **文献名称**: **"Autoantibodies against steroidogenic enzymes in autoimmune Addison's disease"**
**作者**: Brocker, E.B., et al.
**摘要**: 研究探讨了自身免疫性Addison病患者血清中针对类固醇合成途径酶(包括Adrenodoxin)的自身抗体，发现部分患者存在抗Adrenodoxin抗体，提示其可能参与疾病发病机制。
3. **文献名称**: **"Molecular cloning and functional analysis of adrenodoxin reductase–adrenodoxin complexes"**
**作者**: Miller, W.L., et al.
**摘要**: 通过重组技术表达Adrenodoxin并制备单克隆抗体，用于研究其与Adrenodoxin还原酶的相互作用，揭示了电子传递在类固醇生成中的关键作用。
4. **文献名称**: **"Site-directed mutagenesis of adrenodoxin reveals residues critical for its iron-sulfur cluster and antibody binding"**
**作者**: Bernhardt, R., et al.
**摘要**: 利用定点突变技术分析Adrenodoxin结构，结合特异性抗体定位其功能域，阐明铁硫簇结合位点对抗体识别及蛋白功能的影响。
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**注**：上述文献为示例，实际引用时需根据具体数据库(如PubMed)检索确认作者、标题及摘要的准确性。经典研究多集中于Adrenodoxin的生化特性、自身免疫相关性及酶复合体功能分析。
Adrenodoxin (Adx), also known as ferredoxin 1 (FDX1), is a small iron-sulfur protein localized in mitochondria, primarily involved in electron transfer to mitochondrial cytochrome P450 enzymes (CYP11A1. CYP11B1. etc.). These enzymes play critical roles in steroid hormone biosynthesis, including glucocorticoid, mineralocorticoid, and sex hormone production. Adrenodoxin antibodies are immunological tools developed to detect and study this protein's expression, localization, and function in adrenal glands, gonads, and other steroidogenic tissues.
These antibodies are widely used in biomedical research to investigate disorders linked to steroidogenesis, such as congenital adrenal hyperplasia, endocrine cancers, or metabolic diseases. Polyclonal or monoclonal adrenodoxin antibodies are typically generated using recombinant human or bovine Adx proteins or peptide immunogens. They are validated for applications like Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels in tissues or cell models.
Studies leveraging adrenodoxin antibodies have elucidated its role in regulating redox homeostasis and mitochondrial function. Additionally, they aid in exploring Adx's interactions with adrenodoxin reductase and cytochrome P450 systems, providing insights into targeted therapies for hormone-related pathologies. Commercial adrenodoxin antibodies often specify reactivity across species (human, mouse, rat) and require validation in specific experimental contexts to ensure specificity. Their utility extends to clinical diagnostics and drug development for endocrine disorders.
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