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Human Beta-galactoside alpha-2,6-sialyltransferase 1(ST6GAL1) ELISA kit

  • 中文名： 人β半乳糖苷α-2,6-唾液酸转移酶1(ST6GAL1)ELISA Kit
货号: IPD-EL022759HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人β半乳糖苷α-2,6-唾液酸转移酶1(ST6GAL1)酶联免疫试剂盒检测血清、血浆、组织培养上清液、组织匀浆样本中的ST6GAL1含量。ST6GAL1 是重要的靶点。它在细胞生物学中，参与糖基化修饰等过程。研究发现其表达异常与肿瘤的发生、发展、转移及预后密切相关，通过影响肿瘤细胞黏附、侵袭等特性来发挥作用，是肿瘤相关研究的热门靶点。试剂盒检测范围为3.9 ng/mL-250 ng/mL，适用于基础研究中探索ST6GAL1在癌症生物学、免疫微环境调控或神经系统疾病中的功能机制，也可用于药物开发过程中靶向分子表达水平的动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    ST6GAL1; SIAT1; Beta-galactoside alpha-2,6-sialyltransferase 1; Alpha 2,6-ST 1; B-cell antigen CD75; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; ST6Gal I; ST6GalI; Sialyltransferase 1
  • 缩写：
    ST6GAL1
  • Uniprot No.：
    P15907
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    3.9 ng/mL-250 ng/mL
  • 灵敏度：
    0.98 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%





    Three samples of known concentration were tested twenty times on one plate to assess.





    Inter-assay Precision (Precision between assays): CV%<10%





    Three samples of known concentration were tested in twenty assays to assess.












  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ST6GAL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.



    		SampleSerum(n=4)
    1:1Average %92
    Range %86-96

    1:2Average %102
    Range %97-105

    1:4Average %92
    Range %85-98

    1:8Average %97
    Range %91-102

  • 回收率：
    The recovery of human ST6GAL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.












    Sample TypeAverage % RecoveryRange



    Serum (n=5)8680-94



    EDTA plasma (n=4)9792-103

















  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/mlOD1OD2AverageCorrected 2502.8122.9632.8882.731 1252.1572.2032.1802.023 62.51.1581.2561.2071.050 31.250.7150.7550.7350.578 15.60.3990.3950.3970.240 7.80.3020.3220.3120.155 3.90.2420.2320.2370.080 00.1520.1610.157　 

  • 货期：
    3-5 working days

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