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产品描述：猪可溶性P选择素(sP-Selectin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的sP-Selectin含量。sP - Selectin 即可溶性 P - 选择素，它在炎症反应、血栓形成等病理生理过程中扮演重要角色。研究机制上，它可介导白细胞与内皮细胞、血小板的黏附作用，其水平变化能反映机体炎症和血管内皮损伤程度，在心血管疾病等研究中备受关注。试剂盒检测范围为12.5 ng/mL-800 ng/mL，适用于科研领域中对猪心血管疾病模型、炎症机制或免疫调控相关研究，可通过血清/血浆样本评估全身性病理状态，结合细胞培养上清分析体外刺激下的分子释放规律，或通过组织匀浆探究局部微环境中sP-Selectin的表达水平，为动物疾病模型构建、药物作用机制探索及生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：sP-Selectin
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig sP-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 90-96 | |||||
回收率：
| The recovery of pig sP-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 83-90 | ||||
| EDTA plasma (n=4) | 85 | 82-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected8001.8281.9681.8981.7504001.1791.2361.2081.0602000.7340.7570.7460.5981000.4850.4760.4810.333500.3110.3230.3170.169250.2510.2570.2540.10612.50.1880.1820.1850.03700.1450.1510.148 | ||||||
货期：3-5 working days
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