首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠硬骨素(SOST)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SOST含量。SOST是一个重要靶点。它编码的骨硬化蛋白可抑制成骨细胞活性，影响骨形成。目前对其的研究聚焦于揭示调控骨代谢的机制，旨在通过干预SOST，为骨质疏松等骨疾病开发新疗法，改善骨密度和骨质量。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于骨骼发育、骨修复模型构建、代谢性疾病动物实验等科研场景，能够对药物干预、基因编辑或病理状态下SOST的表达水平变化进行动态分析，为骨生物学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sost ELISA Kit; Sclerostin ELISA Kit
缩写：SOST
Uniprot No.：Q99P67
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-99 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 88-94 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 96-103 | |||||
回收率：
| The recovery of rat SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 94-103 | ||||
| EDTA plasma (n=4) | 105 | 105-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.3622.5452.4542.3166001.5881.6861.6371.4993001.1231.1021.1130.9751500.7670.7440.7560.618750.5200.5460.5330.39537.50.3040.3240.3140.17618.750.2480.2410.2450.10700.1360.1400.138 | ||||||
货期：3-5 working days
×
