| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Keratin4; Cytokeratin 4; Keratin-4; CK-4; CK4; KRT14; CYK4 |
| Entrez GeneID | 3851 |
| WB Predicted band size | Calculated MW: 56 kDa; Observed MW: 56 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human Cytokeratin 4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4条关于Cytokeratin 4(CK4)抗体的参考文献示例(内容为模拟生成，非真实文献)：
1. **文献名称**: "Cytokeratin 4 Expression in Normal and Diseased Esophageal Epithelium"
**作者**: Smith A, et al.
**摘要**: 研究通过免疫组化分析CK4在正常食管上皮和Barrett食管中的表达差异，发现CK4在正常复层鳞状上皮中特异性高表达，而在化生组织中表达下调，提示其作为食管疾病诊断标志物的潜力。
2. **文献名称**: "Role of Cytokeratin 4 in Oral Squamous Cell Carcinoma Progression"
**作者**: Lee JH, et al.
**摘要**: 探讨CK4抗体在口腔鳞癌组织中的表达模式，发现CK4表达与肿瘤分化程度相关，低表达与患者预后不良相关，可能作为潜在治疗靶点。
3. **文献名称**: "Development and Validation of a Novel Monoclonal Antibody for Cytokeratin 4"
**作者**: Garcia R, et al.
**摘要**: 报道一种新型抗CK4单克隆抗体的开发，验证其在多种上皮组织中的特异性，并证明其在FFPE(福尔马林固定石蜡包埋)样本中的高敏感性。
4. **文献名称**: "Cytokeratin 4 Interacts with Desmosomal Proteins in Epithelial Integrity"
**作者**: Wang Y, et al.
**摘要**: 通过共聚焦显微镜和免疫沉淀技术，揭示CK4与桥粒蛋白(如Desmoplakin)的相互作用，强调其在维持上皮细胞机械稳定性中的关键作用。
(注：以上文献为示例性质，实际引用需查询PubMed、Web of Science等数据库获取真实文献。)
Cytokeratin 4 (CK4) is a type II intermediate filament protein belonging to the cytokeratin family, which forms structural networks in epithelial cells. As part of the cytoskeleton, cytokeratins provide mechanical stability and resilience to tissues. CK4 is predominantly expressed in stratified squamous epithelia, such as those lining the oral mucosa, esophagus, exocervix, and skin, as well as in certain glandular ducts. It typically pairs with type I cytokeratins (e.g., CK13) to form heterodimers, essential for maintaining epithelial integrity.
Antibodies targeting CK4 are valuable tools in research and diagnostics. In research, they help identify and localize CK4-expressing cells in tissue sections, enabling studies on epithelial differentiation, wound healing, and diseases like squamous cell carcinoma. Clinically, CK4 antibodies are used in immunohistochemistry to distinguish tissue origin in metastatic cancers or to diagnose pathological conditions. For instance, reduced CK4 expression has been linked to Barrett’s esophagus, a precancerous state.
Commercial CK4 antibodies are typically monoclonal or polyclonal, validated for techniques such as Western blotting, immunofluorescence, and IHC. Their specificity ensures accurate detection of CK4 isoforms, aiding in the characterization of epithelial malignancies and developmental biology research. However, interpretation requires caution, as cytokeratin expression can vary with tissue type and disease progression.
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