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Human secretory immunoglobulin A,SIgA ELISA Kit

  • 中文名: 人分泌型免疫球蛋白A(SIgA)ELISA Kit
货号: IPD-E09235h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人分泌型免疫球蛋白A(SIgA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测唾液、尿液、tear样本中的SIgA含量。SIgA即分泌型免疫球蛋白A,是黏膜免疫的关键效应分子。它能在黏膜表面形成保护层,阻止病原体黏附与入侵。相关研究聚焦于其产生机制、与微生物群落互作等,旨在揭示其在抗感染、维持黏膜稳态中的作用,为开发新疗法提供依据。试剂盒检测范围为0.012 μg/mL-50 μg/mL,该产品适用于基础医学研究中黏膜免疫机制探索、感染性疾病模型构建、营养或药物对局部免疫影响的评估等场景,例如通过检测唾液SIgA水平研究口腔免疫应答,或分析泪液SIgA浓度变化探索眼表疾病相关免疫调节机制。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    SIgA
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    saliva, urine, tear
  • 检测范围:
    0.625 μg/ml-40 μg/ml.
  • 灵敏度:
    0.312 μg/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:


  • 标准曲线:


  • 本试剂盒所含材料:



    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human SIgA antibody.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial HRP-conjugated SIgA antibody (100 x concentrate) (120 μl/bottle) ---Bind to the CEA in the samples or standards and react with the substrate to make the solution chromogenic.

    • Two vials Sample Diluent (20 ml/bottle)---Dilute the sample.

    • One vial Wash Buffer (25x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.

    • One vial TMB substrate (10ml/bottle) ---Interact with TMB to elicit a chromogenic reaction.

    • 本试剂盒不含材料:



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期:
        3-5 working days

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