首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肺表面活性物质相关蛋白B(SP-B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFTPB含量。SP-B是肺表面活性物质的重要组成部分，参与调节肺泡表面张力，维持呼吸功能。其研究机制涉及炎症反应、氧化应激，与肺发育障碍密切相关。近年来，深度学习等技术在药物-靶标相互作用预测方面取得进展，为SP-B相关疾病研究提供新思路。试剂盒检测范围为3.12 pg/mL-200 pg/mL，可广泛用于基础科研中肺部疾病机制研究、药物干预效果评估或动物模型（如肺损伤、肺纤维化及新生儿呼吸窘迫综合征）的SP - B动态监测，为探索SP - B在呼吸生理及病理过程中的调控作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
缩写：SFTPB
Uniprot No.：P22355
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 90 | ||||
| Range % | 85-100 | |||||
| 1:2000 | Average % | 94 | ||||
| Range % | 87-98 | |||||
| 1:4000 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:8000 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of rat SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-101 | ||||
| EDTA plasma (n=4) | 91 | 85-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.4702.5892.5302.3771002.1152.1242.1201.967501.7131.7261.7201.567251.3151.3561.3361.18312.50.9850.9710.9780.8256.250.5320.5410.5370.3843.120.3780.3890.3840.23100.1520.1540.153 | ||||||
货期：3-5 working days
×
