| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | P2ry6; P2Y6; P2Y6 receptor; PP2891 |
| Entrez GeneID | 5031 |
| WB Predicted band size | Calculated MW: 36 kDa; Observed MW: 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human P2Y6 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于P2Y6抗体的3篇代表性文献的简要信息，基于公开研究整理：
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1. **文献名称**: "Characterization of a polyclonal antibody against the P2Y6 receptor"
**作者**: Kobayashi K. et al.
**摘要**: 该研究报道了一种兔源多克隆抗体的开发，通过合成P2Y6受体C末端肽段免疫动物获得。抗体经Western blot和免疫组化验证，特异性识别大鼠和小鼠的P2Y6受体，成功应用于脑组织切片中受体的定位研究。
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2. **文献名称**: "P2Y6 receptor-mediated microglial phagocytosis in neuroinflammation"
**作者**: Koizumi S. et al.
**摘要**: 研究利用P2Y6特异性抗体(克隆号未注明)结合流式细胞术和免疫荧光，证明小胶质细胞中P2Y6受体激活后促进β淀粉样蛋白吞噬。抗体特异性通过基因敲除小鼠模型验证，确认其在炎症模型中的功能研究可靠性。
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3. **文献名称**: "Validation of commercial P2Y receptor antibodies using transfected cell lines"
**作者**: Müller C.E. et al.
**摘要**: 文章系统评估了市售P2Y家族(含P2Y6)抗体的特异性。通过转染不同P2Y亚型的HEK293细胞进行交叉实验，发现部分抗体存在非特异性结合，但特定批次的P2Y6抗体(如Santa Cruz, sc-15225)在免疫沉淀中表现良好，为实验选择提供参考。
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**备注**：若需具体文献全文或细节，建议通过PubMed或ResearchGate检索标题/作者，结合机构数据库获取。部分研究可能需关注抗体应用场景(如种属、组织类型)以确保适用性。
The P2Y6 receptor, a member of the purinergic P2Y receptor family, is a G protein-coupled receptor (GPCR) activated by extracellular uridine nucleotides, primarily uridine diphosphate (UDP). It plays critical roles in regulating cellular processes such as inflammation, immune responses, and cell proliferation. The P2Y6 receptor is widely expressed in tissues, including the brain, immune cells, and vascular endothelium, and is involved in signaling pathways linked to phospholipase C (PLC) activation, intracellular calcium mobilization, and MAP kinase cascades. Dysregulation of P2Y6 has been implicated in pathologies like neurodegenerative diseases, atherosclerosis, and cancer.
P2Y6 antibodies are essential tools for studying receptor localization, expression levels, and functional mechanisms. These antibodies, often developed in rabbits or mice, target specific epitopes (e.g., extracellular or intracellular domains) and are validated for techniques such as Western blotting, immunohistochemistry, and flow cytometry. High specificity is ensured through knockout validation. Commercial P2Y6 antibodies enable research into its role in disease models, such as neuroinflammation in Alzheimer’s or macrophage activation in atherosclerosis. Recent studies also explore therapeutic targeting of P2Y6 in chronic inflammatory conditions, highlighting its potential as a biomarker or drug target.
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