首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肺表面活性物质相关蛋白B(SP-B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织培养上清液样本中的SFTPB含量。人肺表面活性物质相关蛋白B（SP-B）是一种由肺泡II型上皮细胞分泌的疏水性蛋白质，主要功能是降低肺泡表面张力，防止肺泡塌陷，维持正常呼吸功能。SP-B的研究机制涉及其在肺表面活性物质复合物中的组装、分泌和重组，以及在不同病理生理情况下的稳态维持。试剂盒检测范围为0.156 ng/mL-10 ng/mL，支持多种生物样本类型检测，适用于体外实验模型中SP-B表达动态监测、疾病动物模型评估、药物干预效果分析等科研场景，为探索肺部病理生理机制及潜在治疗策略提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SFTPB; SFTP3; Pulmonary surfactant-associated protein B; SP-B; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL(Phe
缩写：SFTPB
Uniprot No.：P07988
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, cell culture supernates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 89 | ||||
| Range % | 83-95 | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 87-100 | |||||
| 1:400 | Average % | 88 | ||||
| Range % | 85-91 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-99 | ||||
| EDTA plasma (n=4) | 96 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4532.5632.5082.34752.2282.2612.2452.0842.51.7951.8011.7981.6371.251.2671.2971.2821.1210.6250.8110.8340.8230.6620.3120.5320.5750.5540.3930.1560.3620.3710.3670.20600.1600.1620.161 | ||||||
货期：3-5 working days
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